Cell isolation, expansion and characterization

Cell isolation

To optimize basic and applied biomedical research, Cellider Biotech offers the possibility of customized cell isolation from different tissues including bone marrow, adipose tissue, synovium, dental pulp, cartilage, bone, skin among many others. Tissues can be provided directly from researchers or obtained by Cellider professionals.

Cell expansion

After cell isolation, customers could decide if primary cells are sent to their laboratories or if they prefer their cells to be cultured and expanded under specified culture conditions by the Cellider team. Depending on the cell type, cell expansion may take 1-2 weeks for a single passage. After each passage, 1-2 million cells will be frozen and some others kept in culture upon customer request. Cell growth and proliferation will be recorded. All cell lines are tested for mycoplasma, fungi, and bacteria and guaranteed to be free of any pathogen at the moment of supply.

Cell characterization

Cellider Biotech can characterize cells expanded by Cellider or cells expanded in your laboratory using flow cytometry (analysis of cell surface or intracellular markers), microscopy, or by assessing differentiation capacity to several lineages. For mesenchymal stem cells, approximately two months will be necessary for complete characterization. Surface marker analysis includes the quantification of CD34, CD45, CD44, CD73, CD90, CD105 (any other marker will be included upon request) and at least chondrogenic, osteogenic, and adipogenic differentiation capacity will be performed.

In Cellider Biotech, we adapt our services to your needs.

Please, contact us for detailed information or special requests.

Cellider Biotech

Avda. San Juan Bosco, 13
50009 Zaragoza (Spain)

Phone: +34 876 041 397
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Connect with us

Distributors

1

Avda. San Juan Bosco, 13. 50009 Zaragoza (Spain)

Tel: +34 876 041 397, Mail: info@cellider.com
2

Avda. San Juan Bosco, 13. 50009 Zaragoza (Spain)

Tel: +34 876 041 397, Mail: info@cellider.com